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Journal: Nutrients
Article Title: CHL Attenuates MIA-Induced Osteoarthritis by Regulating Inflammatory, Catabolic, and Anabolic Pathways Associated with Cartilage and Subchondral Bone Degeneration
doi: 10.3390/nu18183031
Figure Lengend Snippet: Effects of CHL on serum biomarkers associated with cartilage degradation and ECM metabolism. SD rats were randomly divided into six groups ( n = 8/group): NC, MIA, MIA + celecoxib (PC, 10 mg/kg/day), and CHL-treated LOW (10 mg/kg/day), MID (30 mg/kg/day), and HIGH (300 mg/kg/day) groups. CHL and celecoxib were administered orally for 6 weeks, with OA induced after 2 weeks of pre-administration by intra-articular injection of MIA. Serum levels of ( A ) COMP, ( B ) CTX-II, ( C ) GAG and ( D ) aggrecan were measured using ELISA kits. Values are presented as the mean ± SD ( n = 8). Statistical significance is indicated by distinct letters, with different letters indicating statistically significant differences between groups. COMP, cartilage oligomeric matrix protein; CTX-II, C-terminal crosslinked telopeptide of type II collagen; and GAG, glycosaminoglycans.
Article Snippet: ELISA kits for GAGs and
Techniques: Injection, Enzyme-linked Immunosorbent Assay
Journal: Bioactive Materials
Article Title: Precisely regulated physically-crosslinked carriers enable synergetic release of bioactive factors for MSC-mediated cartilage regeneration
doi: 10.1016/j.bioactmat.2026.01.009
Figure Lengend Snippet: Biocompatibility and Bioactivity of PSF and KSF in vivo . (a) Transwell Assay of MSCs after treated with PBS, MAP and PSF. Scale bar = 200 μm. (b) Wound Healing Assay of MSCs at 0h and 24h. Scale bar = 200 μm. (c) Immunofluorescent staining of cell pellets after 21 days of co-culture. Blue: DAPI; Green: ActinGreen; Red: Aggrecan. Scale bar = 200 μm. (d) Alcian blue staining of 2D cultured MSCs after 14 days. Scale bar = 200 μm. (e) Cell viability of MSCs at day 3 after co-culture. (f) The cell number of MSCs migrated from upper to lower chamber in Transwell assay. (g) The average distance MSCs migrated from injured margin in wound healing assay. (h–j) qRT-PCR of Col2a1 , Acan and Sox9 mRNA relative expression ratio compared with PBS group. ns: p > 0.05; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Article Snippet: Immunofluorescent staining was performed with
Techniques: In Vivo, Transwell Assay, Wound Healing Assay, Staining, Co-Culture Assay, Cell Culture, Quantitative RT-PCR, Expressing